IgE Protein (AA 209-428) (His-Avi Tag)
Quick Overview for IgE Protein (AA 209-428) (His-Avi Tag) (ABIN7274855)
Target
See all IgE ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 209-428
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Purification tag / Conjugate
- This IgE protein is labelled with His-Avi Tag.
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Purpose
- Human IgE Protein
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Sequence
- Cys209-Lys428
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Characteristics
- Recombinant Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Cys209-Lys428.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IgE, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail.
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IgE (AA 208-429) protein (His-Avi Tag)
Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 91-421) protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 198-421) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IgE
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Background
- Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.
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Molecular Weight
- 27.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
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UniProt
- P01854-1
Target
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