IgG1 Fc (AA 104-330) (Active) Protein

Catalog No. ABIN7274867

Recombinant IgG1 Fc protein expressed in HEK-293 Cells.

Quick Overview for IgG1 Fc (AA 104-330) (Active) Protein (ABIN7274867)

Target: IgG1 Fc

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 104-330

Purpose: Human IgG1 Fc Protein

Sequence: Asp104-Lys330

Characteristics: Recombinant Human IgG1 Fc is expressed from HEK293 without tag.It contains Asp104-Lys330.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).,Human IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Biotinylated Human FcRn, His-Avi Tag on Streptavidin-Biosensor can bind Human IgG1 Fc, No Tag with an affinity constant of 22.8 nM as determined in BLI assay (Gator® Prime).,The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IgG1 Fc

Background: IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Molecular Weight: 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Bis-Tris PAGE result.

UniProt: P01857