IgG2a Fc (AA 98-330) (Active) Protein
This Recombinant IgG2a Fc protein is expressed in HEK-293 Cells.
Catalog No. ABIN7274869
$148.18
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Quick Overview for IgG2a Fc (AA 98-330) (Active) Protein (ABIN7274869)
Target
IgG2a Fc
Protein Type
Recombinant
Biological Activity
Active
Origin
Mouse
Source
HEK-293 Cells
Purity
> 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC
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Protein Characteristics
- AA 98-330
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Purpose
- Mouse IgG2a Fc Protein
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Sequence
- Glu98-Lys330
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Characteristics
- Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).,Mouse IgG2a Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC.
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IgG2a Fc protein (Fc Tag)
Origin: Rat Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IgG2a Fc
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Background
- Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.
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Molecular Weight
- 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result.
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UniProt
- P01863
Target
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