IgG2c Protein (AA 97-330)

Catalog No. ABIN7274870

Recombinant IgG2c protein expressed in HEK-293 Cells.

Quick Overview for IgG2c Protein (AA 97-330) (ABIN7274870)

Target: IgG2c

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 97-330

Purpose: Mouse IgG2C Protein

Sequence: Ile97-Ser330

Characteristics: Recombinant Mouse IgG2C Protein is expressed from HEK293 without tag.It contains Ile97-Ser330.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse IgG2C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgG2C is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IgG2c

Target Type: Antibody

Background: Mouse IgG2c is a subclass of mouse antibody IgG, it can bind FcγRIV with high affinity and the Fc loop residues of mouse IgG2c promote greater receptor-binding affinity than mouse IgG2b or human IgG1.

Molecular Weight: 26.10 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: F6TQW2