IL-10RA Protein (AA 22-233) (His tag)

Catalog No. ABIN7274878

This Recombinant IL-10RA protein is expressed in HEK-293 Cells.

Quick Overview for IL-10RA Protein (AA 22-233) (His tag) (ABIN7274878)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-233

Purification tag / Conjugate: This IL-10RA protein is labelled with His tag.

Purpose: Cynomolgus IL-10 R alpha Protein

Sequence: His22-Asn233

Characteristics: Recombinant Cynomolgus IL-10 R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His22-Asn233.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-10, His Tag with an affinity constant of 0.93 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-10 R alpha, His Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).,Cynomolgus IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: IL-10 R alpha

Background: The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

Molecular Weight: 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5WJ66

Pathways: Growth Factor Binding