IL-15/IL-15R (AA 31-108) (Active) protein (His tag)

Catalog No. ABIN7274889

This Recombinant IL-15/IL-15R protein is produced in HEK-293 Cells.

Quick Overview for IL-15/IL-15R (AA 31-108) (Active) protein (His tag) (ABIN7274889)

Target: IL-15/IL-15R

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-108

Purification tag / Conjugate: His tag

Purpose: Human IL-15RA&IL-15 Protein

Sequence: Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)

Specificity: Uni-Prot: Q13261-1 (IL-15RA), P40933-1 (IL-15)

Characteristics: Recombinant Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test).,Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-15/IL-15R

Alternative Name: IL-15RA&IL-15

Background: Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Molecular Weight: 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Bis-Tris PAGE result.

UniProt: Q13261