IL17 Receptor B Protein (AA 18-292) (His tag)

Catalog No. ABIN7274891

Recombinant IL17 Receptor B protein expressed in HEK-293 Cells.

Quick Overview for IL17 Receptor B Protein (AA 18-292) (His tag) (ABIN7274891)

Target: IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-292

Purification tag / Conjugate: This IL17 Receptor B protein is labelled with His tag.

Purpose: Human IL-17RB Protein

Sequence: Arg18-Per292

Characteristics: Recombinant Human IL-17RB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg18-Per292.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 51.5ng/ml determined by ELISA.,Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.,Human IL-17RB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-17RB is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))

Alternative Name: IL-17RB

Background: Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 (IL-17) cytokine family in malignant diseases. Besides IL-17A, the prototypic member of the IL-17 family, several experimental findings strongly support the role of the IL-17B/IL-17 receptor B (IL-17RB) pathway in tumorigenesis and resistance to anticancer therapies. In mouse models, IL-17B signaling through IL-17RB directly promotes cancer cell survival, proliferation, and migration, and induces resistance to conventional chemotherapeutic agents.

Molecular Weight: 31.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9NRM6