IL-13 Protein (AA 21-132) (His-Avi Tag)

Catalog No. ABIN7274900

Product Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein Type: Recombinant

Protein Characteristics: AA 21-132

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL-13 protein is labelled with His-Avi Tag.

Purpose: Human IL-13 Protein

Sequence: Gly21-Asn132

Characteristics: Recombinant Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.

Purity: > 90 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-13, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 2.7μg/ml determined by ELISA. The affinity constant of 0.7μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL-13 (IL13 Products)

Background: Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Molecular Weight: 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 25-45 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport