IL-13 Protein (AA 26-131) (His tag)

Catalog No. ABIN7320576

Product Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 26-131

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This IL-13 protein is labelled with His tag.

Purpose: Recombinant Mouse Interleukin-13/IL-13 Protein (aa 26-131,His Tag)(Active)

Sequence: Ser26-Phe131

Characteristics: Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Ser26-Phe131 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: Interleukin-13/IL-13 (IL13 Products)

Synonyms: IL13 Protein, IL-13 Protein, P600 Protein, Il-13 Protein, L13 Protein, interleukin 13 Protein, IL13 Protein, Il13 Protein

Background:

Background: Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58 % sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.

Synonym: Interleukin-13, IL-13, T-Cell Activation Protein P600, Il13, Il-13

Molecular Weight: 12.7 kDa

UniProt: P20109

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport