IL-10RA Protein (AA 22-235) (His-Avi Tag)

Catalog No. ABIN7274906

Recombinant IL-10RA protein expressed in HEK-293 Cells.

Quick Overview for IL-10RA Protein (AA 22-235) (His-Avi Tag) (ABIN7274906)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-235

Purification tag / Conjugate: This IL-10RA protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human IL-10 R alpha Protein

Sequence: His22-Asn235

Characteristics: Recombinant Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His22-Asn235.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test).,Human IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: IL-10R alpha

Background: The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

Molecular Weight: 27 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result.

UniProt: Q13651

Pathways: Growth Factor Binding