IL-22 Protein (AA 34-179) (His tag)
Quick Overview for IL-22 Protein (AA 34-179) (His tag) (ABIN7274913)
Target
See all IL-22 (IL22) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 34-179
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Purification tag / Conjugate
- This IL-22 protein is labelled with His tag.
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Purpose
- Cynomolgus IL-22 Protein
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Sequence
- Ala34-Ile179
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Characteristics
- Recombinant Cynomolgus IL-22 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala34-Ile179.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Cynomolgus IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-22, His Tag with an affinity constant of 60.21 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 58.1ng/ml determined by ELISA (QC Test).,Cynomolgus IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IL-22 is greater than 95% as determined by SEC-HPLC.
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Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag)
IL22 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 22 (IL22) (AA 34-179) (Active) protein (Biotin,His-Avi Tag)IL22 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag,AVI tag,Biotin)IL22 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 22 (IL22) protein (mFc Tag)IL22 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 22 (IL22) (AA 27-172) protein (His tag)IL22 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 22 (IL22) (AA 1-179) protein (GST tag)IL22 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag)IL22 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, ELISA, Imm, AcA Active
Interleukin 22 (IL22) (Active) proteinIL22 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL-22 (IL22) (Interleukin 22 (IL22))
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Alternative Name
- IL-22
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Background
- The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
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Molecular Weight
- 17.69 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Bis-Tris PAGE result.
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UniProt
- G7PHZ7
Target
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