IL-22 Protein (AA 34-179) (His tag)
Quick Overview for IL-22 Protein (AA 34-179) (His tag) (ABIN7274919)
Target
See all IL-22 (IL22) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 34-179
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Purification tag / Conjugate
- This IL-22 protein is labelled with His tag.
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Purpose
- Human IL-22 Protein
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Sequence
- Ala34-Ile179
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Characteristics
- Recombinant Human IL-22 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala34-Ile179.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-22, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.,Human IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Human IL-22, His Tag with an affinity constant of 42.60 nM as determined in BLI assay (Gator® Prime).
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Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag)
IL22 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 22 (IL22) (AA 34-179) (Active) protein (Biotin,His-Avi Tag)IL22 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag,AVI tag,Biotin)IL22 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 22 (IL22) protein (mFc Tag)IL22 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 22 (IL22) (AA 27-172) protein (His tag)IL22 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 22 (IL22) (AA 1-179) protein (GST tag)IL22 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag)IL22 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, ELISA, Imm, AcA Active
Interleukin 22 (IL22) (Active) proteinIL22 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL-22 (IL22) (Interleukin 22 (IL22))
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Alternative Name
- IL-22
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Background
- The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
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Molecular Weight
- 17.8 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9GZX6
Target
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