IL-25 Protein (AA 33-177) (His tag)

Catalog No. ABIN7274920

This Recombinant IL-25 protein is expressed in HEK-293 Cells.

Quick Overview for IL-25 Protein (AA 33-177) (His tag) (ABIN7274920)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 33-177

Purification tag / Conjugate: This IL-25 protein is labelled with His tag.

Purpose: Human IL-25/IL-17E Protein

Sequence: Tyr33-Gly177

Characteristics: Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 4.64ug/ml determined by ELISA (QC Test).,Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-25 is greater than 95% and the molecular weight of this protein is around 40-60 kDa as determined by SEC-MALS.,The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL-25

Background: IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Molecular Weight: 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

UniProt: Q9H293