IL-22 Protein (AA 34-179) (Biotin,His-Avi Tag)

Catalog No. ABIN7274927

Recombinant IL-22 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-22 Protein (AA 34-179) (Biotin,His-Avi Tag) (ABIN7274927)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 34-179

Purification tag / Conjugate: This IL-22 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Mouse IL-22 Protein

Sequence: Leu34-Val179

Characteristics: Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).,Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL-22

Background: The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.

Molecular Weight: 19.7 kDa same as Bis-Tris PAGE result.

UniProt: Q9JJY9