IL20RA Protein (AA 33-253) (hFc Tag)

Catalog No. ABIN7274931

This Recombinant IL20RA protein is produced in HEK-293 Cells.

Quick Overview for IL20RA Protein (AA 33-253) (hFc Tag) (ABIN7274931)

Target: IL20RA (Interleukin 20 Receptor alpha (IL20RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 33-253

Purification tag / Conjugate: This IL20RA protein is labelled with hFc Tag.

Purpose: Mouse IL-20RA Protein

Sequence: Val33-Lys253

Characteristics: Recombinant Mouse IL-20RA Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val33-Lys253.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse IL-20RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-20, His Tag with an affinity constant of 13.93 uM as determined in SPR assay (Biacore T200).,Mouse IL-20RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL20RA (Interleukin 20 Receptor alpha (IL20RA))

Alternative Name: IL-20RA

Background: IL-20RA was highly expressed in the tumor tissue of CRC and related to the advanced stage.IL-20RA was involved in regulating oncogenic and immune pathways and affecting the expression of genes related to cell proliferation and immune evasion in CRC.Super-enhancer inhibition by JQ-1 or iBET-151 suppressed the growth of tumor cells and inhibited the expression of IL-20RA.

Molecular Weight: 52.1 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.

UniProt: Q6PHB0