IL23A Protein (AA 22-196) (Biotin,His-Avi Tag)

Catalog No. ABIN7274934

This Recombinant IL23A protein is produced in HEK-293 Cells.

Quick Overview for IL23A Protein (AA 22-196) (Biotin,His-Avi Tag) (ABIN7274934)

Target: IL23A (Interleukin 23, alpha subunit p19 (IL23A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-196

Purification tag / Conjugate: This IL23A protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Mouse IL-23A Protein

Sequence: Val22-Ala196

Characteristics: Recombinant Biotinylated Mouse IL-23A Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Val22-Ala196.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Mouse IL-23A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Mouse IL-23A is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL23A (Interleukin 23, alpha subunit p19 (IL23A))

Alternative Name: IL-23A

Background: The heterodimeric cytokine interleukin-23 (IL-23 or IL23A/IL12B) is produced by dendritic cells and macrophages and promotes the proinflammatory and regenerative activities of T helper 17 (Th17) and innate lymphoid cells. A recent study has reported that IL-23 is also secreted by lung adenoma cells and generates an inflammatory and immune-suppressed stroma.

Molecular Weight: 23.41 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

UniProt: Q9EQ14

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation