IL-33 Protein (AA 112-270)

Catalog No. ABIN7274939

Recombinant IL-33 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-33 Protein (AA 112-270) (ABIN7274939)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 112-270

Purpose: Human IL-33 Protein

Sequence: Ser112-Thr270

Characteristics: Recombinant Human IL-33 Protein is expressed from E.coli without tag.It contains Ser112-Thr270.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-33, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).,Immobilized Human IL-33, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.,Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-33 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Background: Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.

Molecular Weight: 18 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_254274

Pathways: Production of Molecular Mediator of Immune Response