IL-3 Protein (AA 20-152) (Fc Tag)
Quick Overview for IL-3 Protein (AA 20-152) (Fc Tag) (ABIN7274945)
Target
See all IL-3 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 20-152
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Purification tag / Conjugate
- This IL-3 protein is labelled with Fc Tag.
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Purpose
- Human IL-3 Protein
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Sequence
- Ala20-Phe152
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Characteristics
- Recombinant Human IL-3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala20-Phe152.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 0.276 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 20-152) (Active) proteinIL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 3 (IL-3) (AA 20-152) protein (Fc Tag)IL-3 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL-3 (Interleukin 3 (IL-3))
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Alternative Name
- IL-3
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Background
- Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
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Molecular Weight
- 41.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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UniProt
- P08700
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Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
Target
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