IL18BP Protein (AA 31-194) (Biotin,His-Avi Tag)

Catalog No. ABIN7274977

This Recombinant IL18BP protein is expressed in HEK-293 Cells.

Quick Overview for IL18BP Protein (AA 31-194) (Biotin,His-Avi Tag) (ABIN7274977)

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 31-194

Purification tag / Conjugate: This IL18BP protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human IL-18BP Protein

Sequence: Thr31-Gly194

Characteristics: Recombinant Biotinylated Human IL-18BP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr31-Gly194.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human IL-18BP, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag (Cat. IL1-HE018) with an affinity constant of 0.90 nM as determined in SPR assay (Biacore T200).,Biotinylated Human IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Biotinylated Human IL-18BP, His-Avi Tag on Anti-His-Biosensor can bind Human IL-18, No Tag (Cat. IL1-HE018) with an affinity constant of 3.19 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Alternative Name: IL-18BP

Background: Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.

Molecular Weight: 20.5 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.

UniProt: O95998