Interleukin 17a Protein (AA 24-155) (His tag)

Catalog No. ABIN7274991

This Recombinant Interleukin 17a protein is produced in HEK-293 Cells.

Quick Overview for Interleukin 17a Protein (AA 24-155) (His tag) (ABIN7274991)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-155

Purification tag / Conjugate: This Interleukin 17a protein is labelled with His tag.

Purpose: Human IL-17A/CTLA-8 Protein

Sequence: Gly24-Ala155

Characteristics: Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test).,Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA.,Immobilized Human IL-17A, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.,Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA.,Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-17A is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL-17A

Background: Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Molecular Weight: 16.2 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 25-30 kDa based on Bis-Tris PAGE result.

UniProt: Q16552