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Interleukin 17a Protein (AA 26-158) (Biotin,His-Avi Tag)

Recombinant Interleukin 17a protein expressed in HEK-293 Cells.
Catalog No. ABIN7274996
$715.42
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Quick Overview for Interleukin 17a Protein (AA 26-158) (Biotin,His-Avi Tag) (ABIN7274996)

Target

See all Interleukin 17a (IL17A) Proteins
Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 23
  • 12
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Mouse

Source

  • 14
  • 13
  • 7
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Bis-Tris PAGE
  • Protein Characteristics

    AA 26-158

    Purification tag / Conjugate

    This Interleukin 17a protein is labelled with Biotin,His-Avi Tag.

    Purpose

    Biotinylated Mouse IL-17A/CTLA-8 Protein

    Sequence

    Ala26-Ala158

    Characteristics

    Recombinant Biotinylated Mouse IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Ala158.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1 EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Mouse IL-17R alpha at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A, His Tag with the EC50 of 28.3ng/ml determined by ELISA.,Biotinylated Mouse IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

    Alternative Name

    IL-17A

    Background

    Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

    Molecular Weight

    18 kDa. Due to glycosylation, the protein migrates to 19-29 kDa based on Bis-Tris PAGE result.

    UniProt

    Q62386
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