IL22 Receptor alpha 1 Protein (AA 18-225) (His tag)

Catalog No. ABIN7275008

Recombinant IL22 Receptor alpha 1 protein expressed in HEK-293 Cells.

Quick Overview for IL22 Receptor alpha 1 Protein (AA 18-225) (His tag) (ABIN7275008)

Target: IL22 Receptor alpha 1 (IL22RA1) (Interleukin 22 Receptor, alpha 1 (IL22RA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-225

Purification tag / Conjugate: This IL22 Receptor alpha 1 protein is labelled with His tag.

Purpose: Cynomolgus IL-22R alpha 1 Protein

Sequence: Pro18-Thr225

Characteristics: Recombinant Cynomolgus IL-22R alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro18-Thr225.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-22, His Tag with an affinity constant of 60.21 nM as determined in SPR assay (Biacore T200).,Cynomolgus IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL22 Receptor alpha 1 (IL22RA1) (Interleukin 22 Receptor, alpha 1 (IL22RA1))

Alternative Name: IL-22R alpha 1

Background: IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF).Component of the receptor for IL20, IL22 and IL24. Component of IL22 receptor formed by IL22RA1 and IL10RB enabling IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. Mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.

Molecular Weight: 24.84 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5TKX9