IL27 Receptor alpha Protein (AA 34-516) (hFc Tag)

Catalog No. ABIN7275016

This Recombinant IL27 Receptor alpha protein is expressed in HEK-293 Cells.

Quick Overview for IL27 Receptor alpha Protein (AA 34-516) (hFc Tag) (ABIN7275016)

Target: IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 34-516

Purification tag / Conjugate: This IL27 Receptor alpha protein is labelled with hFc Tag.

Purpose: Human IL-27RA/TCCR Protein

Sequence: Gly34-Lys516

Characteristics: Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly34-Lys516.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test).,Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))

Alternative Name: IL-27 Ra

Background: A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.

Molecular Weight: 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.

UniProt: Q6UWB1

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response