IL-22R alpha 1 & IL-10R beta (AA 16-228) (Active) protein (hFc-Avi Tag,Biotin)

Catalog No. ABIN7275039

This Recombinant IL-22R alpha 1 & IL-10R beta protein is produced in HEK-293 Cells.

Quick Overview for IL-22R alpha 1 & IL-10R beta (AA 16-228) (Active) protein (hFc-Avi Tag,Biotin) (ABIN7275039)

Target: IL-22R alpha 1 & IL-10R beta

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 16-228

Purification tag / Conjugate: hFc-Avi Tag,Biotin

Purpose: Biotinylated Human IL-22R alpha 1&IL-10R beta Protein

Sequence: His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta)

Specificity: Uni-Prot: Q8N6P7 (IL-22R alpha 1), Q08334 (IL-10R beta)

Characteristics: Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from HEK293 with hFc (IgG1) tag and Avi Tag at the C-Terminus of IL-22R alpha 1 and IL-10R beta.It contains His16-Thr228 (IL-22R alpha 1) and Met20-Ser220 (IL-10R beta).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.77ug/ml determined by ELISA.,Biotinylated Human IL-22R alpha 1&IL-10R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human IL-22, His Tag on Anti-His-Biosensor can bind Biotinylated Human IL-22R alpha 1&IL-10R beta, hFc-Avi Tag with an affinity constant of 13.70 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human IL-22R alpha 1&IL-10R beta is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: IL-22R alpha 1 & IL-10R beta

Background: IL-22Rα1&IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL--22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 Rβ or IL-20 Rβ and the respective ligands is required for signal transduction[1].

Molecular Weight: 52.07 kDa (IL-22R alpha 1) and 48.85 kDa (IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.

UniProt: Q8N6P7