IL22 Receptor alpha 1 Protein (AA 16-228) (hFc Tag)

Catalog No. ABIN7275045

This Recombinant IL22 Receptor alpha 1 protein is produced in HEK-293 Cells.

Quick Overview for IL22 Receptor alpha 1 Protein (AA 16-228) (hFc Tag) (ABIN7275045)

Target: IL22 Receptor alpha 1 (IL22RA1) (Interleukin 22 Receptor, alpha 1 (IL22RA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 16-228

Purification tag / Conjugate: This IL22 Receptor alpha 1 protein is labelled with hFc Tag.

Purpose: Mouse IL-22R alpha 1 Protein

Sequence: His16-Ala228

Characteristics: Recombinant Mouse IL-22R alpha 1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains His16-Ala228.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.,Mouse IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).,The purity of Mouse IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL22 Receptor alpha 1 (IL22RA1) (Interleukin 22 Receptor, alpha 1 (IL22RA1))

Alternative Name: IL-22R alpha 1

Background: Interleukin (IL)-22 is a member of the IL-10 family of cytokines and represents an important effector molecule of activated Th22, Th1, and Th17 cells, as well as Tc-cell subsets, gammadelta T cells, natural killer (NK), and NKT cells. IL-22 mediates its effects via a heterodimeric transmembrane receptor complex consisting of IL-22R1 and IL-10R2 and subsequent Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathways including Jak1, Tyk2, and STAT3.

Molecular Weight: 51.1 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.

UniProt: Q80XZ4