Integrin alpha X beta 2 (ITGAX & ITGB2) Heterodimer (AA 20-1107), (Heterodimer) (Active) protein (His tag)

Catalog No. ABIN7275054

This Recombinant Integrin alpha X beta 2 (ITGAX & ITGB2) Heterodimer protein is produced in HEK-293 Cells.

Quick Overview for Integrin alpha X beta 2 (ITGAX & ITGB2) Heterodimer (AA 20-1107), (Heterodimer) (Active) protein (His tag) (ABIN7275054)

Target: Integrin alpha X beta 2 (ITGAX & ITGB2) Heterodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-1107, Heterodimer

Purification tag / Conjugate: His tag

Purpose: Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein

Sequence: Phe20-Pro1107(ITGAX) acidic tail & Gln23-Asn700(ITGB2) basic tail

Specificity: Uni-Prot: P20702-1 (ITGAX), P05107-1 (ITGB2)

Characteristics: Recombinant Human Integrin alpha X beta 2(ITGAX&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGAX.It contains Phe20-Pro1107(ITGAX) acidic tail and Gln23-Asn700(ITGB2) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ITGAX&ITGB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).,Human ITGAX&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ITGAX&ITGB2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Integrin alpha X beta 2 (ITGAX & ITGB2) Heterodimer

Background: The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response.

Molecular Weight: 125.50 kDa (ITGAX) and 79.50 kDa (ITGB2). Due to glycosylation, the protein migrates to 130-135 kDa (ITGAX) and 85-95 kDa (ITGB2) based on Bis-Tris PAGE result.

UniProt: P20702