KIR2DL2 Protein (AA 22-245) (Biotin,His-Avi Tag)

Catalog No. ABIN7275087

This Recombinant KIR2DL2 protein is produced in HEK-293 Cells.

Quick Overview for KIR2DL2 Protein (AA 22-245) (Biotin,His-Avi Tag) (ABIN7275087)

Target: KIR2DL2 (Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 2 (KIR2DL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-245

Purification tag / Conjugate: This KIR2DL2 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human KIR2DL2 Protein

Sequence: His22-His245

Characteristics: Recombinant Biotinylated Human KIR2DL2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His22-His245.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human KIR2DL2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated-Human KIR2DL2 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KIR2DL2 (Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 2 (KIR2DL2))

Alternative Name: KIR2DL2

Background: KIR2DL2 (2DL2, formerly NKAT6, designated CD158b) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig-like receptor (KIR) family. KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system.KIR2DL2 is receptor on natural killer (NK) cells for HLA-Cw1, 3, 7, and 8 allotypes. Inhibits the activity of NK cells thus preventing cell lysis.

Molecular Weight: 27.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: P43627