KLKB1 Protein (AA 20-638) (His tag)

Catalog No. ABIN7275094

This Recombinant KLKB1 protein is expressed in HEK-293 Cells.

Quick Overview for KLKB1 Protein (AA 20-638) (His tag) (ABIN7275094)

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-638

Purification tag / Conjugate: This KLKB1 protein is labelled with His tag.

Purpose: Cynomolgus KLKB1 Protein (pro form)

Sequence: Gly20-Ala638

Characteristics: Recombinant Cynomolgus KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Gly20-Ala638.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus KLKB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is > 800 pmol/min/ug (QC Test).,The purity of Cynomolgus KLKB1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Alternative Name: KLKB1

Background: Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.

Molecular Weight: 70.19 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5VTJ9

Pathways: Complement System, Brown Fat Cell Differentiation