KLRG1 Protein (AA 57-188) (hFc Tag)

Catalog No. ABIN7275100

This Recombinant KLRG1 protein is expressed in HEK-293 Cells.

Quick Overview for KLRG1 Protein (AA 57-188) (hFc Tag) (ABIN7275100)

Target: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 57-188

Purification tag / Conjugate: This KLRG1 protein is labelled with hFc Tag.

Purpose: Mouse KLRG1 Protein

Sequence: Gln57-Tyr188

Characteristics: Recombinant Mouse KLRG1 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Gln57-Tyr188.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse KLRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse KLRG1, hFc Tag on ProA-Biosensor, can bind Human E-Cadherin, His Tag with an affinity constant of 0.70 μM as determined in BLI assay (Gator).,The purity of Mouse KLRG1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Alternative Name: KLRG1

Background: Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine.

Molecular Weight: 42.3 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.

UniProt: O88713