KIR3DL2 Protein (AA 22-339) (His-Avi Tag)

Catalog No. ABIN7275102

This Recombinant KIR3DL2 protein is produced in HEK-293 Cells.

Quick Overview for KIR3DL2 Protein (AA 22-339) (His-Avi Tag) (ABIN7275102)

Target: KIR3DL2 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 2 (KIR3DL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-339

Purification tag / Conjugate: This KIR3DL2 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human KIR3DL2 Protein

Sequence: Leu22-Leu339

Characteristics: Recombinant Human KIR3DL2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu22-Leu339.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human KIR3DL2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-KIR3DL2 Antibody, hFc Tag with the EC50 of 47.8ng/ml determined by ELISA.,Human KIR3DL2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human KIR3DL2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KIR3DL2 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 2 (KIR3DL2))

Alternative Name: KIR3DL2

Background: KIR3DL2 is a member of the killer cell immunoglobulin-like receptor (KIR) family that was initially identified at the surface of natural killer (NK) cells. KIR3DL2, also known as CD158k, is expressed as a disulfide-linked homodimer. Each chain is composed of three immunoglobulin-like domains and a long cytoplasmic tail containing two immunoreceptor tyrosine-based inhibitory motifs.

Molecular Weight: 37.9 kDa. Due to glycosylation, the protein migrates to 40-70 kDa based on Bis-Tris PAGE result.

UniProt: P43630