LDLR Protein (AA 22-788) (His-Avi Tag)

Catalog No. ABIN7275139

This Recombinant LDLR protein is expressed in HEK-293 Cells.

Quick Overview for LDLR Protein (AA 22-788) (His-Avi Tag) (ABIN7275139)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-788

Purification tag / Conjugate: This LDLR protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human LDLR Protein

Sequence: Ala22-Arg788

Characteristics: Recombinant Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala22-Arg788.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200).,Human LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human LDLR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Alternative Name: LDLR

Background: The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.

Molecular Weight: 87.6 kDa, Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism