TNFSF14 Protein (AA 89-240, Trimer) (His-DYKDDDDK Tag)

Catalog No. ABIN7275148

This Recombinant TNFSF14 protein is expressed in HEK-293 Cells.

Quick Overview for TNFSF14 Protein (AA 89-240, Trimer) (His-DYKDDDDK Tag) (ABIN7275148)

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 89-240, Trimer

Purification tag / Conjugate: This TNFSF14 protein is labelled with His-DYKDDDDK Tag.

Purpose: Human LIGHT/TNFSF14 Trimer Protein

Sequence: Ser89-Val240

Characteristics: Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus., It contains Ser89-Val240 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).,Human LIGHT Trimer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 90%.,The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Alternative Name: LIGHT

Background: LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.

Molecular Weight: 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: O43557

Pathways: Cancer Immune Checkpoints