LIF Protein (AA 25-203) (Fc Tag)
Quick Overview for LIF Protein (AA 25-203) (Fc Tag) (ABIN7275154)
Target
See all LIF ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 25-203
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Purification tag / Conjugate
- This LIF protein is labelled with Fc Tag.
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Purpose
- Mouse LIF Protein
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Sequence
- Pro25-Phe203
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Characteristics
- Recombinant Mouse LIF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Pro25-Phe203.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- LIF (Leukemia Inhibitory Factor (LIF))
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Alternative Name
- LIF
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Background
- Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
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Molecular Weight
- 46.5 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.
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UniProt
- P09056
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Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Target
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