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LILRB4 Protein (AA 22-259) (His tag)

This Recombinant LILRB4 protein is produced in HEK-293 Cells.

SDS

Immobilized Cynomolgus LILRB4, His Tag at 5 μg/mL (100 μL/well) on the plate. (LILRB4 Protein (AA 22-259) (His tag))

ELISA

SEC-HPLC

ELISA

4 images

Catalog No. ABIN7275159

$445.92

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Quick Overview for LILRB4 Protein (AA 22-259) (His tag) (ABIN7275159)

Target

LILRB4 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))

Protein Type

Recombinant

Biological Activity

Active

Origin

Cynomolgus

Source

HEK-293 Cells

Purity

> 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Protein Characteristics

AA 22-259

Purification tag / Conjugate

This LILRB4 protein is labelled with His tag.

Purpose

Cynomolgus LILRB4/CD85k/ILT3 Protein

Sequence

Gln22-Glu259

Characteristics

Recombinant Cynomolgus LILRB4/CD85k/ILT3 Protein Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Glu259.

Sterility

0.22 μm filtered

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity Comment

Immobilized Cynomolgus LILRB4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.,Cynomolgus LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus LILRB4 is greater than 95% as determined by SEC-HPLC.
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Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-256) (Active) protein (hFc Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-257) (Active) protein (His-Avi Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-257) (Active) protein (Biotin,His-Avi Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 1-448) protein (GST tag)
LILRB4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-118) (Active) protein (His-Avi Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 119-259) (Active) protein (Biotin,His-Avi Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-118) (Active) protein (Biotin,His-Avi Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) protein (His tag)
LILRB4 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-259) protein (Fc Tag)
LILRB4 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 24-206) protein (His tag)
LILRB4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % WB, SDS, Imm, PC

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage

-20 °C,-80 °C

Storage Comment

-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date

12 months
Target

LILRB4 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))

Alternative Name

LILRB4

Background

LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

Molecular Weight

27.2 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.

NCBI Accession

XP_015297198