LILRA4 Protein (AA 24-446) (His-Avi Tag)

Catalog No. ABIN7275179

Recombinant LILRA4 protein expressed in HEK-293 Cells.

Quick Overview for LILRA4 Protein (AA 24-446) (His-Avi Tag) (ABIN7275179)

Target: LILRA4 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 4 (LILRA4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-446

Purification tag / Conjugate: This LILRA4 protein is labelled with His-Avi Tag.

Purpose: Human LILRA4/CD85g Protein

Sequence: Glu24-Asn446

Characteristics: Recombinant Human LILRA4/CD85g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Asn446.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LILRA4 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 4 (LILRA4))

Alternative Name: LILRA4

Background: LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain.LILRA4 function coreceptor to limit the innate immune responses to viral infections, signaling occurs via FCER1G (PubMed:16735691, PubMed:19564354). Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as negative regulator of TLR7 and TLR9 signaling cascades.

Molecular Weight: 49.5 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.

UniProt: P59901