LRRN1 Protein (AA 26-631) (His tag)

Catalog No. ABIN7275196

This Recombinant LRRN1 protein is expressed in HEK-293 Cells.

Quick Overview for LRRN1 Protein (AA 26-631) (His tag) (ABIN7275196)

Target: LRRN1 (Leucine Rich Repeat Neuronal 1 (LRRN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-631

Purification tag / Conjugate: This LRRN1 protein is labelled with His tag.

Purpose: Mouse LRRN1 Protein

Sequence: Ser26-Ala631

Characteristics: Recombinant Mouse LRRN1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser26-Ala631.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse LRRN1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse LRRN1 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRRN1 (Leucine Rich Repeat Neuronal 1 (LRRN1))

Alternative Name: LRRN1

Background: Lrrn1 is required for the formation of MHB--loss of function leads to a loss of the morphological constriction and loss of Fgf8. Cells overexpressing Lrrn1 violate the boundary and result in a loss of cell restriction between midbrain and hindbrain compartments. Lrrn1 also regulates the glycosyltransferase Lunatic Fringe, a modulator of Notch signalling, maintaining its expression in midbrain cells which is instrumental in MHB boundary formation.

Molecular Weight: 69.28 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result.

UniProt: Q61809