Lrig1 Protein (AA 34-792) (His tag)

Catalog No. ABIN7275204

This Recombinant Lrig1 protein is produced in HEK-293 Cells.

Quick Overview for Lrig1 Protein (AA 34-792) (His tag) (ABIN7275204)

Target: Lrig1 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 34-792

Purification tag / Conjugate: This Lrig1 protein is labelled with His tag.

Purpose: Cynomolgus LRIG1 Protein

Sequence: Ala34-Thr792

Characteristics: Recombinant Cynomolgus LRIG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala34-Thr792.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus LRIG1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-LRIG1 Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.,Cynomolgus LRIG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Lrig1 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))

Alternative Name: LRIG1

Background: The leucine-rich repeats and immunoglobulin-like domains (LRIG)-1 is a tumor suppressor gene that belongs to the LRIG family. LRIG1 expression has prognostic significance in various human cancers. Somatic mutations, which are associated with a certain rate of response to targeted therapies, are ubiquitously found in human non-small cell lung cancer (NSCLC). LRIG1 was an independent prognostic factor for OS of NSCLC patients. LRIG1 in combination with other clinicopathological risk factors was a stronger prognostic model than clinicopathological risk factors alone.

Molecular Weight: 84.16 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5V8J9