LRP5 Protein (AA 644-1263) (mFc Tag)

Catalog No. ABIN7275209

Recombinant LRP5 protein expressed in HEK-293 Cells.

Quick Overview for LRP5 Protein (AA 644-1263) (mFc Tag) (ABIN7275209)

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 644-1263

Purification tag / Conjugate: This LRP5 protein is labelled with mFc Tag.

Purpose: Human LRP-5 Protein

Sequence: Glu644-Gln1263

Characteristics: Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Glu644-Gln1263.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.20μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP-5

Background: A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.

Molecular Weight: 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.

UniProt: O75197-1

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation