LRP5 Protein (AA 644-1263) (mFc Tag)
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- Target See all LRP5 Proteins
- LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 644-1263
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LRP5 protein is labelled with mFc Tag.
- Purpose
- Human LRP-5 Protein
- Sequence
- Glu644-Gln1263
- Characteristics
- Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Glu644-Gln1263.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.20μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product LRP5 Protein
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Low Density Lipoprotein Receptor-Related Protein 5 (LRP5) (AA 644-1263) protein (Fc Tag)
LRP5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Low Density Lipoprotein Receptor-Related Protein 5 (LRP5) (AA 644-1263) (Active) protein (Fc Tag)LRP5 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Low Density Lipoprotein Receptor-Related Protein 5 (LRP5) (AA 41-130) protein (GST tag)LRP5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
- Alternative Name
- LRP-5 (LRP5 Products)
- Synonyms
- Xlrp5 Protein, bmnd1 Protein, evr1 Protein, evr4 Protein, hbm Protein, lr3 Protein, lrp5-A Protein, lrp7 Protein, oppg Protein, ops Protein, opta1 Protein, vbch2 Protein, LRP5 Protein, LRP6 Protein, BMND1 Protein, EVR1 Protein, EVR4 Protein, HBM Protein, LR3 Protein, LRP7 Protein, OPPG Protein, OPS Protein, OPTA1 Protein, VBCH2 Protein, mKIAA4142 Protein, low-density lipoprotein receptor-related protein 5 Protein, LDL receptor related protein 5 Protein, LDL receptor related protein 5 L homeolog Protein, low density lipoprotein receptor-related protein 5 Protein, LDL receptor related protein 5 like Protein, LOC100147353 Protein, LRP5 Protein, lrp5.L Protein, lrp5 Protein, Lrp5 Protein, LRP5L Protein
- Background
- A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.
- Molecular Weight
- 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
- Pathways
- WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation
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