LRRC15 Protein (AA 22-538) (His-Avi Tag)

Catalog No. ABIN7275215

Recombinant LRRC15 protein expressed in CHO Cells.

Quick Overview for LRRC15 Protein (AA 22-538) (His-Avi Tag) (ABIN7275215)

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-538

Purification tag / Conjugate: This LRRC15 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human LRRC15/LIB Protein

Sequence: Tyr22-Gly538

Characteristics: Recombinant Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LRRC15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.,Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Alternative Name: LRRC15

Background: The leucine-rich repeat-containing protein 15 (LRRC15) is involved in cell-cell and cell-matrix interactions and came into focus as a promising anticancer target owing to its overexpression in mesenchymal-derived tumors such as sarcoma, glioblastoma, and melanoma and in cancer-associated fibroblasts in the microenvironment of breast, head and neck, lung, and pancreatic tumors.

Molecular Weight: 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: Q8TF66