LTBR Protein (AA 31-223) (hFc Tag)

Catalog No. ABIN7275219

This Recombinant LTBR protein is produced in HEK-293 Cells.

Quick Overview for LTBR Protein (AA 31-223) (hFc Tag) (ABIN7275219)

Target: LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-223

Purification tag / Conjugate: This LTBR protein is labelled with hFc Tag.

Purpose: Mouse LTBR Protein

Sequence: Gln31-Leu223

Characteristics: Recombinant Mouse LTBR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln31-Leu223.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse LTBR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse LTBR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))

Alternative Name: LTBR

Background: Components of lymphotoxin beta receptor (LTBR)-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB. TRAF3 functions as a negative regulator of LTBR signaling via both canonical and non-canonical NFkappaB pathways by two distinct mechanisms. These effects of TRAF3 depletion did not require LTBR signaling and were consistent with autonomous activation of the non-canonical NFkappaB pathway.

Molecular Weight: 48.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: P50284

Pathways: NF-kappaB Signaling