MFAP4 Protein (AA 60-293) (His tag)
Quick Overview for MFAP4 Protein (AA 60-293) (His tag) (ABIN7275229)
Target
See all MFAP4 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 60-293
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Purification tag / Conjugate
- This MFAP4 protein is labelled with His tag.
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Purpose
- Cynomolgus MFAP4 Protein
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Sequence
- Val60-Ala293
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Characteristics
- Recombinant Cynomolgus MFAP4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val60-Ala293.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Cynomolgus MFAP4, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.,Cynomolgus MFAP4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus MFAP4 is greater than 95% as determined by SEC-HPLC.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 24 months
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- MFAP4 (Microfibrillar-Associated Protein 4 (MFAP4))
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Alternative Name
- MFAP4
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Background
- Microfibril-associated glycoprotein 4 (MFAP4) is an extracellular matrix protein belonging to the fibrinogen-related protein superfamily. MFAP4 is produced by vascular smooth muscle cells and is highly enriched in the blood vessels of the heart and lung, where it is thought to contribute to the structure and function of elastic fibers. Genetic studies in humans have implicated MFAP4 in the pathogenesis of Smith-Magenis syndrome, in which patients present with multiple congenital abnormalities and mental retardation, as well as in the severe cardiac malformation left-sided congenital heart disease.
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Molecular Weight
- 27.60 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
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UniProt
- A0A2K5TQS8
Target
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