MFAP5 Protein (AA 29-164) (hFc Tag)

Catalog No. ABIN7275233

Recombinant MFAP5 protein expressed in HEK-293 Cells.

Quick Overview for MFAP5 Protein (AA 29-164) (hFc Tag) (ABIN7275233)

Target: MFAP5 (Microfibrillar Associated Protein 5 (MFAP5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-164

Purification tag / Conjugate: This MFAP5 protein is labelled with hFc Tag.

Purpose: Mouse MFAP5 Protein

Sequence: Gln29-Leu164

Characteristics: Recombinant Mouse MFAP5 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln29-Leu164.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse MFAP5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse MFAP5 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MFAP5 (Microfibrillar Associated Protein 5 (MFAP5))

Alternative Name: MFAP5

Background: Human basal-like breast cancer (BLBC) is an aggressive malignancy with poor prognosis. Since most current treatments are ineffective, there is an urgent need to identify therapeutic targets for BLBC. Microfibrillar-associated protein 5 (MFAP5) plays an important role in the integration of elastic microfibers and the regulation of endothelial cell behaviors.MFAP5 was significantly overexpressed in BLBC tissues and associated with poor metastasis-free survival of patients with BLBC.

Molecular Weight: 42.4 kDa. Due to furin cleavage and glycosylation, the protein migrates to 32-38 kDa and 50-65 kDa based on Bis-Tris PAGE result.

UniProt: Q9QZJ6