MCEMP1 Protein (AA 92-183) (hFc Tag)

Catalog No. ABIN7275236

Recombinant MCEMP1 protein expressed in HEK-293 Cells.

Quick Overview for MCEMP1 Protein (AA 92-183) (hFc Tag) (ABIN7275236)

Target: MCEMP1 (Mast Cell Expressed Membrane Protein 1 (MCEMP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 92-183

Purification tag / Conjugate: This MCEMP1 protein is labelled with hFc Tag.

Purpose: Mouse MCEMP1 Protein

Sequence: Lys92-Thr183

Characteristics: Recombinant Mouse MCEMP1 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Lys92-Thr183.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse MCEMP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse MCEMP1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MCEMP1 (Mast Cell Expressed Membrane Protein 1 (MCEMP1))

Alternative Name: MCEMP1

Background: A cecal ligation and puncture-induced sepsis mouse model was established to determine the expression of mast cell expression membrane protein 1 (MCEMP1). MCEMP1 in T lymphocytes isolated from sepsis mice were up- or downregulated by exogenous transfection in an attempt to investigate their effects on the release of inflammatory factors, the expression of immunoglobulins, the activity of T cell subsets and natural killer (NK) cells, as well as T lymphocyte apoptosis.

Molecular Weight: 37.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q9D8U6