MEPE Protein (AA 25-441) (His tag)

Catalog No. ABIN7275245

Recombinant MEPE protein expressed in HEK-293 Cells.

Quick Overview for MEPE Protein (AA 25-441) (His tag) (ABIN7275245)

Target: MEPE (Matrix Extracellular phosphoglycoprotein (MEPE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 25-441

Purification tag / Conjugate: This MEPE protein is labelled with His tag.

Purpose: Mouse MEPE Protein

Sequence: Ala25-Asp441

Characteristics: Recombinant Mouse MEPE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Asp441.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse MEPE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MEPE (Matrix Extracellular phosphoglycoprotein (MEPE))

Alternative Name: MEPE

Background: Matrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.

Molecular Weight: 45.3 kDa. The protein is cleaved into 2 chains and due to glycosylation, it migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: Q8K4L6