MFI2 Protein (AA 20-709) (His tag)

Catalog No. ABIN7275252

This Recombinant MFI2 protein is produced in HEK-293 Cells.

Quick Overview for MFI2 Protein (AA 20-709) (His tag) (ABIN7275252)

Target: MFI2 (Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-709

Purification tag / Conjugate: This MFI2 protein is labelled with His tag.

Purpose: Human CD228/MFI2 Protein

Sequence: Gly20-Cys709

Characteristics: Recombinant Human CD228/MFI2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys709.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).,Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CD228 Ab., hFc-Avi Tag on ProA-Biosensor can bind Human CD228, His Tag with an affinity constant of 1.54 nM as determined in BLI assay (Gator® Prime).,The purity of Human CD228 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MFI2 (Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2))

Alternative Name: CD228

Background: Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.

Molecular Weight: 76.60 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Bis-Tris PAGE result.

UniProt: P08582

Pathways: Transition Metal Ion Homeostasis, Cell-Cell Junction Organization