Mesothelin Protein (MSLN) (AA 406-690) (His tag,Biotin)

Catalog No. ABIN7275281

Product Details

Target: Mesothelin (MSLN)

Protein Type: Recombinant

Protein Characteristics: AA 406-690

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This Mesothelin protein is labelled with His tag,Biotin.

Purpose: Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling)

Sequence: Asp406-Gly690

Characteristics: Recombinant Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp406-Gly690.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus MSLN, His Tag with the EC50 of 54.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Mesothelin (MSLN)

Alternative Name: MSLN (MSLN Products)

Background: Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31- kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Molecular Weight: 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005590874

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process