Mesothelin Protein (MSLN) (AA 296-580) (Biotin,His-Avi Tag)

Catalog No. ABIN7275286

This Recombinant Mesothelin protein is expressed in HEK-293 Cells.

Quick Overview for Mesothelin Protein (MSLN) (AA 296-580) (Biotin,His-Avi Tag) (ABIN7275286)

Target: Mesothelin (MSLN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 296-580

Purification tag / Conjugate: This Mesothelin protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human MSLN/Mesothelin Protein

Sequence: Glu296-Gly580

Characteristics: Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu296-Gly580.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-MSLN Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, His Tag with the EC50 of 13.7ng/ml determined by ELISA.,Biotinylated Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human MSLN was greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Mesothelin (MSLN)

Alternative Name: MSLN

Background: Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31- kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Molecular Weight: 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process