MCAM Protein (AA 24-559) (His tag)
Quick Overview for MCAM Protein (AA 24-559) (His tag) (ABIN7275297)
Target
See all MCAM ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 24-559
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Purification tag / Conjugate
- This MCAM protein is labelled with His tag.
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Purpose
- Human MUC18/CD146 Protein
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Sequence
- Val24-Gly559
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Characteristics
- Recombinant Human MUC18/CD146 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Gly559.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human MUC18, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
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Melanoma Cell Adhesion Molecule (MCAM) (AA 24-563) protein (His tag)
MCAM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) protein (GST tag)MCAM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-646) protein (His-DYKDDDDK-Strep II Tag)MCAM Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Melanoma Cell Adhesion Molecule (MCAM) protein (His tag)MCAM Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)MCAM Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)MCAM Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Alternative Name
- MUC18
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Background
- METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous, actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells.
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Molecular Weight
- 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
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UniProt
- P43121
Target
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