MUC16 Protein (AA 12660-12923) (His-Avi Tag)

Catalog No. ABIN7275299

Recombinant MUC16 protein expressed in HEK-293 Cells.

Quick Overview for MUC16 Protein (AA 12660-12923) (His-Avi Tag) (ABIN7275299)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 12660-12923

Purification tag / Conjugate: This MUC16 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human CA125/MUC16 Protein

Sequence: Gly12660-Met12923

Characteristics: Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly12660-Met12923.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.13ug/ml determined by ELISA.,Human CA125 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human CA125, His-Avi Tag on Anti-His-Biosensor can bind Human MSLN, hFc Tag with an affinity constant of 31.00 nM as determined in BLI assay (Gator® Prime).,The purity of Human CA125 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125

Background: MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.

Molecular Weight: 31.3 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

UniProt: Q8WXI7